Translucent appearance described the opacity of the colony meaning that it was almost clear but distorted (Kysela, Randich, Caccamo, & Brun, 2016). Gray defined the bacterial pigmentation during growth in the medium. The flat characteristic described the side view elevation of the colony while smooth implies that the margins of the colony are entire. The bacterial colony description was beta-hemolytic meaning that the organism lyses red cells entirely using hemolysins O and S (cytotoxins) produced by the bacteria. There were clear zones observed in the growth medium after the addition of iodine. Finally, the Gram-positive bacteria grew on starch agar after inoculation thus, tested positive for starch hydrolysis test. subtilis which both test positive for VP stain and negative for MR test (Sneath, Mair, Sharpe, & Holt, 1986). The second test was MR/VP staining which tested negative affirming that the bacteria was B. The outcomes observed included green spores and pink rod-like structures. The first test was spore staining whereby the bacteria tested positive, showing the ability to grow spores. This experiment pursued the Gram-positive rods whereby, additional stains and tests were used to determine the unknown. Colony description are as follows: Description The initial findings included observations of the colony in the growth media and recorded as Side A and B. Finally, all the reactions used to test for the unknown gram-positive rods were recorded on a log sheet to aid in the writing of a lab report. My lab partner inoculated the identified gram-negative rods on an API 20 E strip and motility agar isolated. Additional tests (spore staining, starch hydrolysis test, and Methyl Red/ Voges Proskauer (MR/VP) test), inoculation on starch agar, and Voges–Proskauer (VP) test was done for Gram-positive rods that were identified during the procedure. The following step was to follow the directions on the appropriate flow chart from lab notes and manual to identify the appropriate tests to carry out. In the third step, the instructor confirmed the Gram reaction before proceeding with the inoculation steps. The second step involved a Gram Stain reaction procedure, determination of bacterial morphology, and the arrangement observed. The first step was describing the bacterial colony using the terms provided in the laboratory handout by indicating as side A or B. Methyl Red and Voges Proskauer stains were elemental in identifying the species of bacilli bacteria that are gram-positive and exhibits a rod shape. A starch agar helped in identifying whether the bacteria could hydrolyze glucose in the agar. A spore test kit was instrumental in determining whether the bacteria sporulated. A Gram Stain was used in elucidating the Gram reaction, arrangement, and morphology of the bacterial colony. Various materials were critical for the successful identification of the unknown. The primary goal of the experiment was to identify unknown bacteria using specific guidelines and procedures of identifying and differentiating bacterial species. Therefore, the utilization of specific tests and procedures were instrumental in bacterial identification and analysis. For instance, the MR/VP test was used to determine the fermentation pathway that the unknown bacteria used to utilize glucose while starch hydrolysis test identified whether the bacterial species of interest could hydrolyze amylase and amylopectin using alpha-amylase and oligo-1,6-glucosidase enzymes thus, differentiating the genera Bacillus and Clostridium. Identifying the bacterial morphology, arrangement and gram reaction were pivotal to narrowing down the bacterial species and carrying specific tests that would help identify the specific bacteria. The experiment was conducted to identify bacterial species based on the gram stain reaction, morphology, and arrangement of the isolated colonies in the growth medium. Identification and Analysis of Bacillus megaterium
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